Cullin 3 Antibody (JE37-29)

Western Blot: Cullin 3 Antibody (JE37-29) [NBP3-32232]

Western Blot: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Western blot analysis of Cullin 3 on different lysates with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution.

Lane 1: SW480 cell lysate
Lane 2: SW620 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Rat testis tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 89 kDa
Observed band size: 75 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32232) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cullin 3 Antibody (JE37-29) [NBP3-32232]

Immunohistochemistry: Cullin 3 Antibody (JE37-29) [NBP3-32232] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cullin 3 antibody (NBP3-32232) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32232) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.