Cullin 4a Antibody (5P4M1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16539
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5P4M1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cullin 4a Antibody (5P4M1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 660-759 of human Cullin 4a (Q13619). FKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cullin 4a Antibody (5P4M1)
![Western Blot: Cullin 4a Antibody (5P4M1) [NBP3-16539]](https://resources.bio-techne.com/images/products/Cullin-4a-Antibody-5P4M1-Western-Blot-NBP3-16539-img0002.jpg "Western Blot: Cullin 4a Antibody (5P4M1) [NBP3-16539]")
Western Blot: Cullin 4a Antibody (5P4M1) [NBP3-16539]
Western Blot: Cullin 4a Antibody (5P4M1) [NBP3-16539] - Western blot analysis of extracts of various cell lines, using Cullin 4a Rabbit mAb (NBP3-16539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.![Immunohistochemistry-Paraffin: Cullin 4a Antibody (5P4M1) [NBP3-16539]](https://resources.bio-techne.com/images/products/Cullin-4a-Antibody-5P4M1-Immunohistochemistry-Paraffin-NBP3-16539-img0001.jpg "Immunohistochemistry-Paraffin: Cullin 4a Antibody (5P4M1) [NBP3-16539]")
Immunohistochemistry-Paraffin: Cullin 4a Antibody (5P4M1) [NBP3-16539]
Immunohistochemistry-Paraffin: Cullin 4a Antibody (5P4M1) [NBP3-16539] - Immunohistochemistry of paraffin-embedded human colon using Cullin 4a Rabbit mAb (NBP3-16539) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Cullin 4a Antibody (5P4M1)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CUL4A
Long Name
Cullin 4a
Alternate Names
Cullin 4a
Gene Symbol
CUL4A
Additional CUL4A Products
Product Documents for Cullin 4a Antibody (5P4M1)
Product Specific Notices for Cullin 4a Antibody (5P4M1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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