Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CUL4A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 759) of human Cullin 4A using the numbering given in entry NP_001008895.1 (GeneID 8451).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cullin 4a Antibody

Western Blot: Cullin 4a Antibody [NB100-2267]

Western Blot: Cullin 4a Antibody [NB100-2267]

Western Blot: Cullin 4a Antibody [NB100-2267] - Detection of Human and Mouse Cul4a by WB (h&m) and Immunoprecipitation (h). Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Cul4a antibody NB100-2267 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Cul4a was also immunoprecipitated by rabbit anti-Cul4a antibody NB100-2266.
Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267]

Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267]

Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267] - Section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Cul4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for Cullin 4a Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CUL4A

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitinconjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Long Name

Cullin 4a

Alternate Names

Cullin 4a

Entrez Gene IDs

8451 (Human); 99375 (Mouse)

Gene Symbol

CUL4A

UniProt

Q13619 (Human)

Additional CUL4A Products

Product Documents for Cullin 4a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cullin 4a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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