Cullin 4a Antibody - N-terminal

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78040

Novus Biologicals, part of Bio-Techne
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NBP1-78040
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CUL4A Antibodies »

Product Specifications

Immunogen

This Cullin 4a Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cullin 4a coupled to KLH. (Uniprot: Q13619)

Reactivity Notes

This product reacts with human Cullin 4a. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cullin 4a Antibody - N-terminal

Western Blot: Cullin 4a AntibodyN-terminal [NBP1-78040]

Western Blot: Cullin 4a AntibodyN-terminal [NBP1-78040]

Western Blot: Cullin 4a Antibody [NBP1-78040] - Western Blot of Cullin 4a antibody. Lane 1: Opal Pre-Stained Ladder. Lane 2: human Testis. Lane 3: human skeletal muscle. Load: 35ug lysate per lane. Primary Antibody: Anti-Cullin 4a at 1:500 overnight at 2-8C. Secondary Antibody: Goat anti-rabbit IgG HRP at 1:70,000 at RT for 30 mins. Expect: ~87.6kDa.
Immunohistochemistry: Cullin 4a Antibody - N-terminal [NBP1-78040]

Immunohistochemistry: Cullin 4a Antibody - N-terminal [NBP1-78040]

Immunohistochemistry: Cullin 4a Antibody [NBP1-78040] - Immunohistochemistry. Cullin 4a antibody was diluted 1:500 to detect Cullin 4a in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
N/A: Cullin 4a Antibody [NBP1-78040]
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Applications for Cullin 4a Antibody - N-terminal

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:500

Western Blot

1:500-1:1000
Application Notes
This product has been tested by western blot, immunoprecipitation, and immunohistochemistry. The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others). This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1. An 87.6 kDa band corresponding to human Cul4A is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

Antiserum

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: CUL4A

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Long Name

Cullin 4a

Alternate Names

Cullin 4a

Gene Symbol

CUL4A

UniProt

Q13619 (Human), AAR13072 (Human)

Additional CUL4A Products

Product Documents for Cullin 4a Antibody - N-terminal

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cullin 4a Antibody - N-terminal

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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