Cullin 4a Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38019
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-300 of human Cullin 4a (NP_001008895.1).
Sequence:
IERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLT
Sequence:
IERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cullin 4a Antibody
Immunoprecipitation: Cullin 4a Antibody [NBP3-38019] -
Immunoprecipitation: Cullin 4a Antibody [NBP3-38019] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug Cullin 4a antibody. Western blot was performed from the immunoprecipitate using Cullin 4a antibody at a dilution of 1:1000.
Western Blot: Cullin 4a Antibody [NBP3-38019] -
Western Blot: Cullin 4a Antibody [NBP3-38019] - Western blot analysis of various lysates using Cullin 4a Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Applications for Cullin 4a Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CUL4A
Long Name
Cullin 4a
Alternate Names
Cullin 4a
Gene Symbol
CUL4A
Additional CUL4A Products
Product Documents for Cullin 4a Antibody
Product Specific Notices for Cullin 4a Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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