Cullin-7 Antibody - C-terminus

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78042

Novus Biologicals, part of Bio-Techne
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NBP1-78042
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cullin-7 Antibodies »

Product Specifications

Immunogen

This Cullin-7 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus region near amino acids 1675-1698 of Human Cullin-7 coupled to KLH. (Uniprot: Q14999)

Reactivity Notes

Cross reactivity with other human cullins may occur

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cullin-7 Antibody - C-terminus

Immunohistochemistry: Cullin-7 Antibody - C-terminus [NBP1-78042]

Immunohistochemistry: Cullin-7 Antibody - C-terminus [NBP1-78042]

Immunohistochemistry: Cullin-7 Antibody [NBP1-78042] - Cullin-7 antibody was diluted 1:500 to detect Cullin-7 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
N/A: Cullin-7 Antibody [NBP1-78042]
Cullin-7 Antibody - C-terminus

Cullin-7 Antibody - C-terminus

Anti-CUL7 antibody was diluted 1:500 to detect CUL7 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Applications for Cullin-7 Antibody - C-terminus

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:1000
Application Notes
This product has been tested by immunohistochemistry. This antibody reacts with human and mouse Cul7 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and also immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). This antibody also co-immunoprecipitates associated proteins in overexpressed CUL7 systems. A 191.2 kDa band corresponding to human Cul7 is detected. Most cell lines expressing Cul7 can be used as a positive control. Researchers should determine optimal titers for other applications.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

Antiserum

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Cullin-7

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

Alternate Names

CUL-7, cullin 7, cullin-7, KIAA0076dJ20C7.5

Gene Symbol

CUL7

UniProt

NP_055595 (Human), Q14999 (Human)

Additional Cullin-7 Products

Product Documents for Cullin-7 Antibody - C-terminus

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cullin-7 Antibody - C-terminus

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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