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CXCL14/BRAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31398

Novus Biologicals, part of Bio-Techne
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NBP1-31398

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CXCL14/BRAK. The exact sequence is proprietary.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCL14/BRAK Antibody

Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry: CXCL14/BRAK Antibody [NBP1-31398] - Two-color immunofluorescence staining of primary colon cancer tissue with anti-CXCL14 and BerEP4. (A) Anti-CXCL14 red color. (C) Overlays giving yellow color of double-stained areas (BerEP4, green color). (H) Rabbit IgG; negative control for anti-CXCL14. Original magnification: 200x. Image collected and cropped by CiteAb from the following publication (mdpi.com/1422-0067/20/22/5793) licensed under a CC-BY license.
Western Blot: CXCL14/BRAK Antibody [NBP1-31398]

Western Blot: CXCL14/BRAK Antibody [NBP1-31398]

Western Blot: CXCL14/BRAK Antibody [NBP1-31398] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL14 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Frozen: CXCL14/BRAK Antibody [NBP1-31398]

Immunohistochemistry-Frozen: CXCL14/BRAK Antibody [NBP1-31398] - Human lung tissue stained for CXCL14 (red) counterstained with DAPI. IHC-Fr image submitted by a verified customer review.

Applications for CXCL14/BRAK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 28775895).

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:100-1:1000

Knockdown Validated

Reported in scientific literature (PMID: 28775895).

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-31398 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CXCL14/BRAK

The CXCL14 gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this s

Alternate Names

Bolekine, BRAK, CXC-X3

Gene Symbol

CXCL14

Additional CXCL14/BRAK Products

Product Documents for CXCL14/BRAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCL14/BRAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.