CXCL7/NAP-2 Antibody (3B9)
Novus Biologicals, part of Bio-Techne | Catalog # H00005473-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Knockdown Validated, Sandwich ELISA, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3B9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPBP (AAH28217, 37 a.a. ~ 128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TKGQTKRNLAKGKEESLDSDLYAELRCMCIKTTSGIHPKNIQSLEVIGKGTHCNQVEVIATLKDGRKICLDPDAPRIKKIVQKKLAGDESAD
Specificity
PPBP - pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for CXCL7/NAP-2 Antibody (3B9)
![Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01]](https://resources.bio-techne.com/images/products/CXCL7-NAP-2-Antibody-3B9-Western-Blot-H00005473-M01-img0006.jpg "Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01]")
Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01]
Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.![Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01]](https://resources.bio-techne.com/images/products/CXCL7-NAP-2-Antibody-3B9-Western-Blot-H00005473-M01-img0004.jpg "Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01]")
Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01]
Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi ( Cat # H00005473-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 (Cat # H00005473-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Detection limit for recombinant GST tagged PPBP is approximately 0.03ng/ml as a capture antibody.
Applications for CXCL7/NAP-2 Antibody (3B9)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA. IHC-Fr usage reported in scientific literature (PMID: 25858640).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CXCL7/NAP-2
Alternate Names
CTAP III, NAP-2, NAP2, PPBP
Entrez Gene IDs
5473 (Human)
Gene Symbol
PPBP
OMIM
121010 (Human)
UniProt
Additional CXCL7/NAP-2 Products
Product Documents for CXCL7/NAP-2 Antibody (3B9)
Product Specific Notices for CXCL7/NAP-2 Antibody (3B9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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