CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12005
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free [NBP3-12005]](https://resources.bio-techne.com/images/products/CXCR2-IL-8RB-Antibody-HC2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12005-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free [NBP3-12005]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # HC2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing mice with human IL-8 (interleukin-8) receptor, type B.
Specificity
HC2 recognizes human C-X-C chemokine receptor type 2, (CXCR2). CD182 is a 360 amino acid multipass membrane glcycoprotein and is expressed by neutrophils and monocytes, as well as by some subsets of T cells and endothelial cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free [NBP3-12005]
Immunocytochemistry/Immunofluorescence: CXCR2/IL-8RB Antibody (HC2) - Chimeric [NBP3-12005] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HC2 (NBP3-12005) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12005, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CXCR2/IL-8RB
Long Name
Interleukin 8 Receptor B
Alternate Names
CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB
Gene Symbol
CXCR2
Additional CXCR2/IL-8RB Products
Product Documents for CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free
Product Specific Notices for CXCR2/IL-8RB Antibody (HC2) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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