Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry

Label

Janelia Fluor 646

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 358426

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CXCR7/RDC-1 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
Met1-Lys362 (Gly131Ser)
Accession # AAA62370

Specificity

Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for CXCR7/RDC-1 Antibody (358426) [Janelia Fluor® 646]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CXCR7/RDC-1

CXCR7/RDC-1 is a G protein-coupled receptor (GPCR) member of the CXC subfamily of chemokine receptors. Human CXCR7/RDC-1 is 362 amino acids (aa) in length with a predicted molecular weight of 41 kDa. Mouse and rat CXCR7/RDC-1 share 93% aa sequence identity with the human protein. CXCR7/RDC-1 binds to and acts as a scavenger for CXCL11/I-TAC and CXCL12/SDF-1. CXCR7/RDC-1 can also function as a co-receptor for HIV and SIV. Unlike other chemokine receptors, CXCR7/RDC-1 does not activate G protein signaling, but instead initiates beta-Arrestin-mediated receptor-ligand internalization. Although CXCR7/RDC-1 itself does not activate G protein signaling, the receptor can heterodimerize with CXCR4 to activate G proteins in response to CXCL12/SDF-1 binding. Studies on CXCR7/RDC-1 knockout mice suggest that it is critical for cardiovascular development. While CXCR7/RDC-1 does not appear to signal in normal hematopoietic cells, it is highly expressed in leukemic cells where it activates Akt signaling that promotes cell trafficking and adhesion. CXCR7/RDC-1 also mediates neuronal migration, displays aberrant signaling in astrocytes, and is highly expressed in glioma cells.

Alternate Names

ACKR3, CMKOR1, CXCR7, GPR159, RDC-1

Gene Symbol

CXCR7

Additional CXCR7/RDC-1 Products

Product Documents for CXCR7/RDC-1 Antibody (358426) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CXCR7/RDC-1 Antibody (358426) [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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