CYC1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86872
![Western Blot: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Western-Blot-NBP1-86872-img0007.jpg "Western Blot: CYC1 Antibody [NBP1-86872]")
![Western Blot: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Western-Blot-NBP1-86872-img0013.jpg "Western Blot: CYC1 Antibody [NBP1-86872]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYR
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25605331).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CYC1 Antibody
![Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86872-img0011.jpg "Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]")
Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]
Immunocytochemistry/Immunofluorescence: CYC1 Antibody [NBP1-86872] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.![Immunohistochemistry: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Immunohistochemistry-NBP1-86872-img0018.jpg "Immunohistochemistry: CYC1 Antibody [NBP1-86872]")
Immunohistochemistry: CYC1 Antibody [NBP1-86872]
Immunohistochemistry: CYC1 Antibody [NBP1-86872] - Tissue localization and semi-quantification of the mitochondrial respiratory enzyme complexes in the murine distal colon during C. rodentium infection. Immunohistochemical staining using antibodies for CYC1 (complex-III). Statistics: unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control, n = 5-6 mice/group. The infection experiments were performed twice, and each time point contain results pooled from 5-6 mice. Scale bar 100 um, magnification x 200. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep15434), licensed under a CC-BY license.![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0014.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0015.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0016.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
![Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]](https://resources.bio-techne.com/images/products/CYC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86872-img0017.jpg "Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]")
Applications for CYC1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-P reported in scientific literature (PMID: 26481427). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 4 using NBP1-86872 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYC1
Alternate Names
Complex III subunit 4, Complex III subunit IV, cytochrome c1, heme protein, mitochondrial, cytochrome c-1Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, UQCR4
Gene Symbol
CYC1
Additional CYC1 Products
Product Documents for CYC1 Antibody
Product Specific Notices for CYC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...