Cyclin B1 Antibody (V92.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2648

Novus Biologicals, part of Bio-Techne
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NB100-2648
NB100-2648-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V92.1

Format

BSA Free

Concentration

1 mg/ml

View all Cyclin B1 Antibodies »

Product Summary for Cyclin B1 Antibody (V92.1) - BSA Free

Immunogen

Hamster Cyclin B1

Localization

Nuclear

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Cyclin B1 Antibody (V92.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1.0 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1 - 2 ug/500ug protein

Western Blot

1:100 - 1:2000
Application Notes
Precipitates active CDK1/Cyclin B1 complexesThis antibody is Cytof ready. Positive control(s): Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin B1

In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Alternate Names

CCNB1

Entrez Gene IDs

891 (Human); 268697 (Mouse)

Gene Symbol

CCNB1

Additional Cyclin B1 Products

Product Documents for Cyclin B1 Antibody (V92.1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclin B1 Antibody (V92.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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