Cyclin B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33031

Novus Biologicals, part of Bio-Techne
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NBP1-33031
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cyclin B2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin B2. The exact sequence is proprietary.

Specificity

human CCNB2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin B2 Antibody

Western Blot: Cyclin B2 Antibody [NBP1-33031]

Western Blot: Cyclin B2 Antibody [NBP1-33031]

Western Blot: Cyclin B2 Antibody [NBP1-33031] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody [NBP1-33031]

Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody [NBP1-33031]

Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody [NBP1-33031] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: CCNB2 protein stained by Cyclin B2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031]

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031]

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Cyclin B2 Antibody detects Cyclin B2 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Cyclin B2 Antibody diluted at 1:500.
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Applications for Cyclin B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclin B2

Cyclin B2 belongs to a the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin B2 has been shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins.

Alternate Names

CCNB2

Gene Symbol

CCNB2

Additional Cyclin B2 Products

Product Documents for Cyclin B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cyclin B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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