Cyclin D3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92937

Novus Biologicals, part of Bio-Techne
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NBP2-92937-0.02ml
NBP2-92937-0.1ml
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin D3 Antibodies »

Product Summary for Cyclin D3 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human Cyclin D3 (NP_001751.1). SMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin D3 Antibody - Azide and BSA Free

Western Blot: Cyclin D3 AntibodyAzide and BSA Free [NBP2-92937]

Western Blot: Cyclin D3 AntibodyAzide and BSA Free [NBP2-92937]

Western Blot: Cyclin D3 Antibody [NBP2-92937] - Analysis of extracts of various cell lines, using Cyclin D3 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Knockout Validated: Cyclin D3 Antibody - Azide and BSA Free [NBP2-92937]

Knockout Validated: Cyclin D3 Antibody - Azide and BSA Free [NBP2-92937]

Knockout Validated: Cyclin D3 Antibody [NBP2-92937] - Analysis of extracts from normal (control) and CCND3 knockout (KO) HeLa cells, using Cyclin D3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in

Applications for Cyclin D3 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cyclin D3

During the G1 phase of the cell cycle, a cell must "choose" between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.

Alternate Names

CCND3

Gene Symbol

CCND3

Additional Cyclin D3 Products

Product Documents for Cyclin D3 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclin D3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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