Cyclin D3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98894

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin D3 Antibodies »

Product Summary for Cyclin D3 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Cyclin D3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cyclin D3 Antibody

Western Blot: Cyclin D3 Antibody [NBP2-98894]

Western Blot: Cyclin D3 Antibody [NBP2-98894]

Western Blot: Cyclin D3 Antibody [NBP2-98894] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa
Immunoprecipitation: Cyclin D3 Antibody [NBP2-98894]

Immunoprecipitation: Cyclin D3 Antibody [NBP2-98894]

Immunoprecipitation: Cyclin D3 Antibody [NBP2-98894] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-CCND3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CCND3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 36 kDa

Applications for Cyclin D3 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin D3

During the G1 phase of the cell cycle, a cell must "choose" between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.

Alternate Names

CCND3

Gene Symbol

CCND3

Additional Cyclin D3 Products

Product Documents for Cyclin D3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin D3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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