Cyclin E1 Antibody (5F8C5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37247
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5F8C5
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human Cyclin E1 (AA: 307-410) expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cyclin E1 Antibody (5F8C5) - BSA Free
Western Blot: Cyclin E1 Antibody (5F8C5)BSA Free [NBP2-37247]
Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.Flow Cytometry: Cyclin E1 Antibody (5F8C5) - BSA Free [NBP2-37247]
Flow Cytometry: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).Western Blot: Cyclin E1 Antibody (5F8C5)BSA Free [NBP2-37247]
Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)Applications for Cyclin E1 Antibody (5F8C5) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Western Blot
1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cyclin E1
Alternate Names
CCNE, CCNE1
Gene Symbol
CCNE1
Additional Cyclin E1 Products
Product Documents for Cyclin E1 Antibody (5F8C5) - BSA Free
Product Specific Notices for Cyclin E1 Antibody (5F8C5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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