Cyclin E1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16055

Novus Biologicals, part of Bio-Techne
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NBP2-16055
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cyclin E1 Antibodies »

Product Summary for Cyclin E1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Cyclin E1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%), Bovine (86%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin E1 Antibody

Western Blot: Cyclin E1 Antibody [NBP2-16055]

Western Blot: Cyclin E1 Antibody [NBP2-16055]

Western Blot: Cyclin E1 Antibody [NBP2-16055] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cyclin E1 Antibody [NBP2-16055]

Immunocytochemistry/ Immunofluorescence: Cyclin E1 Antibody [NBP2-16055]

Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody [NBP2-16055] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cyclin E1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Western Blot: Cyclin E1 Antibody [NBP2-16055]

Western Blot: Cyclin E1 Antibody [NBP2-16055]

Western Blot: Cyclin E1 Antibody [NBP2-16055] - Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for Cyclin E1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclin E1

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq]

Alternate Names

CCNE, CCNE1

Gene Symbol

CCNE1

UniProt

P24864 (Human)

Additional Cyclin E1 Products

Product Documents for Cyclin E1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin E1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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