Cyclin J Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90019

Novus Biologicals, part of Bio-Techne
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NBP1-90019
NBP1-90019-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cyclin J Antibodies »

Product Summary for Cyclin J Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TRLHRLTAYSWDFLVQCIERLLIAHDNDVKEANKQRGQAGPQSAQLSVFQTASQPSRPVHFQQPQYLHQTHQTSLQYRHPT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin J Antibody

Western Blot: Cyclin J Antibody [NBP1-90019]

Western Blot: Cyclin J Antibody [NBP1-90019]

Western Blot: Cyclin J Antibody [NBP1-90019] - Analysis in control (vector only transfected HEK293T lysate) and CCNJ over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Cyclin J Antibody [NBP1-90019]

Immunohistochemistry-Paraffin: Cyclin J Antibody [NBP1-90019]

Immunohistochemistry-Paraffin: Cyclin J Antibody [NBP1-90019] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal and non-germinal center cells.

Applications for Cyclin J Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin J

CCNJ( AAH43175, 1 a.a. - 373 a.a.) full-length recombinant protein with GST.

Alternate Names

bA690P14.1, cyclin J, cyclin-J, FLJ10895

Entrez Gene IDs

54619 (Human)

Gene Symbol

CCNJ

UniProt

Q5T5M9 (Human)

Additional Cyclin J Products

Product Documents for Cyclin J Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin J Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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