Cyclin L2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-87009

Novus Biologicals, part of Bio-Techne
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NB100-87009
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoblotting, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunoblotting, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Cyclin L2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 470 and 520 of human cyclin L2 using the numbering given in entry NP_112199.2 (GeneID 81669).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin L2 Antibody

Western Blot: Cyclin L2 Antibody [NB100-87009]

Western Blot: Cyclin L2 Antibody [NB100-87009]

Western Blot: Cyclin L2 Antibody [NB100-87009] - Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Cyclin L2 Antibody [NB100-87009]

Immunoprecipitation: Cyclin L2 Antibody [NB100-87009]

Immunoprecipitation: Cyclin L2 Antibody [NB100-87009] - Detection of human Cyclin L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Cyclin L2 antibody NB100-87009 (lot 2) used for IP at 6 ug per reaction. Cyclin L2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-Cyclin L2 antibody from Company B. For blotting immunoprecipitated Cyclin L2, NB100-87009 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Cyclin L2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Knockdown validation (PMID: 31852782). Use in immunoblotting reported in scientific literature (PMID:31852782).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cyclin L2

Cyclin L2 is a member of the cyclin family of regulatory proteins that associate with and activate cyclin-dependent kinases (CDKs) to regulate cell cycle progression and transcription. The hallmark characteristic of cyclins is the periodicity of their expression and activity that is regulated transcriptionally and post-transcriptionally via phosphorylation and ubiquitination. Cyclin L2 is part of a group of cyclins that are involved in transcriptional regulation at the level of RNA transcription, initiation, elongation, and RNA processing. The transcriptional regulating cyclins include cyclin C, H, K, L1, L2, T1, and T2 which associate with CDK7, -8, -9, -10 and -12. Cyclin L2 associates with CDK11 and RNA polymerase II and co-localizes with splicing factors and is implicated to play a role in pre-mRNA splicing. Alternate names for cyclin L2 include cyclin M, paneth cell-enhanced expression protein, CCNM, and CCNL2.

Alternate Names

ania-6b, CCNM, cyclin L2, cyclin M, cyclin-L2, DKFZp761A1210, DKFZp762O195, HCLA-ISO, HLA-ISO, Paneth cell-enhanced expression protein, PCEE, SB138

Entrez Gene IDs

81669 (Human)

Gene Symbol

CCNL2

UniProt

Q96S94 (Human)

Additional Cyclin L2 Products

Product Documents for Cyclin L2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin L2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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