CYFIP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50028
![Western Blot: CYFIP1 Antibody [NBP1-50028]](https://resources.bio-techne.com/images/products/CYFIP1-Antibody-Western-Blot-NBP1-50028-img0003.jpg "Western Blot: CYFIP1 Antibody [NBP1-50028]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for CYFIP1 Antibody
Immunogen
The immunogen for this product maps to a region between residue 1203 and 1253 of human Cytoplasmic FMR1 Interacting Protein 1 using the numbering given in entry NP_055423.1 (GeneID 23191).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CYFIP1 Antibody
Western Blot: CYFIP1 Antibody [NBP1-50028]
Western Blot: CYFIP1 Antibody [NBP1-50028] - Detection of Human CYFIP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CYFIP1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CYFIP1 was also immunoprecipitated by rabbit anti-CYFIP1 antibody BL10812, which recognizes an upstream epitope. For blotting immunoprecipitated CYFIP1, the ReliaBLOT. Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for CYFIP1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CYFIP1
Alternate Names
cytoplasmic FMR1 interacting protein 1, cytoplasmic FMR1-interacting protein 1, FLJ45151, KIAA0068cytoplasmic FMRP interacting protein 1, p140sra-1, selective hybridizing clone, SHYC, Specifically Rac1-associated protein 1, SRA1, sra-1
Gene Symbol
CYFIP1
Additional CYFIP1 Products
Product Documents for CYFIP1 Antibody
Product Specific Notices for CYFIP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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