CYLD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33589

Novus Biologicals, part of Bio-Techne
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NBP2-33589
NBP2-33589-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYLD Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPLEINSRVSLKVGETIESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRFDGVQLCSFACVESTILLHINDIIPESVTQER

Reactivity Notes

Rat (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYLD Antibody

Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NBP2-33589]

Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NBP2-33589]

Immunocytochemistry/Immunofluorescence: CYLD Antibody [NBP2-33589] - Staining of human cell line A-431 shows localization to microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-33589]

Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-33589]

Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-33589] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Applications for CYLD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYLD

Ubiquitin carboxyl-terminal hydrolase CYLD (CYLD) is a 956 amino acid (aa) member of the peptidase C67 protein family with a predicted molecular weight of 107 kDa. The mouse and rat CYLD orthologs share 95% and 94% aa sequence identity with the human protein, respectively. Two isoforms of CYLD have been identified, a full-length isoform and a second isoform that lacks aa 305-307 due to alternative splicing. Expression of CYLD has been reported in fetal brain as well as adult brain, heart, leukocytes, skeletal muscle, spleen, and testis. CYLD acts as a deubiquitinase and removes K63-linked Ubiquitin chains from multiple substrates including IkB, c-Jun, and c-Fos, resulting in the inhibition of NFkB and JNK signaling. In some contexts, CYLD enhances mitosis entry, and it has also been shown to delay G 1 /S phase entry suggesting that CYLD regulates multiple phases of the cell cycle. CYLD is recognized as a tumor suppressor and mutations in CYLD result in skin appendage syndromes including Brooke-Spiegle Syndrome, familial cylindromatosis, and familial trichoepitheliomas type 1.

Long Name

Cylindromatosis

Alternate Names

BRSS, CDMT, CYLD1, EAC, MFT1, SBS, TEM, USPL2

Gene Symbol

CYLD

UniProt

Q9NQC7 (Human)

Additional CYLD Products

Product Documents for CYLD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYLD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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