CYP11A1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92879

Novus Biologicals, part of Bio-Techne
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NBP2-92879-0.02ml
NBP2-92879-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CYP11A1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of CYP11A1 (NP_000772.2). MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYP11A1 Antibody - Azide and BSA Free

Western Blot: CYP11A1 AntibodyAzide and BSA Free [NBP2-92879]

Western Blot: CYP11A1 AntibodyAzide and BSA Free [NBP2-92879]

Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of HeLa cells, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry: CYP11A1 Antibody - Azide and BSA Free [NBP2-92879]

Immunohistochemistry: CYP11A1 Antibody - Azide and BSA Free [NBP2-92879]

Immunohistochemistry: CYP11A1 Antibody [NBP2-92879] - Immunofluorescence analysis of human placenta using CYP11A1 Rabbit pAb (NBP2-92879) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: CYP11A1 AntibodyAzide and BSA Free [NBP2-92879]

Western Blot: CYP11A1 AntibodyAzide and BSA Free [NBP2-92879]

Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of various cell lines, using CYP11A1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
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Applications for CYP11A1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-92879 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYP11A1

Alternate Names

Cholesterol desmolase, cholesterol monooxygenase (side-chain-cleaving), cholesterol side-chain cleavage enzyme, mitochondrial, CYPXIA1, Cytochrome P450 11A1, Cytochrome P450(scc), cytochrome P450, family 11, subfamily A, polypeptide 1, cytochrome P450C11A1, EC 1.14.15, EC 1.14.15.6, steroid 20-22-lyase, subfamily XIA (cholesterol side chain cleavage)

Gene Symbol

CYP11A1

Additional CYP11A1 Products

Product Documents for CYP11A1 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CYP11A1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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