CYP21A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38698

Novus Biologicals, part of Bio-Techne
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NBP2-38698
NBP2-38698-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYP21A2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP21A2 Antibody

Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698]

Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698]

Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Analysis in human adrenal gland and kidney tissues using NBP2-38698 antibody. Corresponding CYP21A2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698]

Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698]

Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland, kidney, pancreas and testis using Anti-CYP21A2 antibody NBP2-38698 (A) shows similar protein distribution across tissues to independent antibody NBP2-13893 (B).
Western Blot: CYP21A2 Antibody [NBP2-38698]

Western Blot: CYP21A2 Antibody [NBP2-38698]

Western Blot: CYP21A2 Antibody [NBP2-38698] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue
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Applications for CYP21A2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western internal validation: Separated by size; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP21A2

The CYP21A2 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins aremonooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids andother lipids. This protein localiz

Long Name

Cytochrome P450 Family 21 Subfamily A Member 2

Alternate Names

21-OHase, CAH1, CPS1, CYP21, CYP21B

Gene Symbol

CYP21A2

UniProt

P08686 (Human)

Additional CYP21A2 Products

Product Documents for CYP21A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYP21A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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