CYP24A1 Antibody (HL1783) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25427
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1783
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human CYP24A1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CYP24A1 Antibody (HL1783) - Azide and BSA Free
Immunohistochemistry-Paraffin: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] -
Immunohistochemistry-Paraffin: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - CYP24A1 antibody [HL1783] detects CYP24A1 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. CYP24A1 stained by CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Knockdown Validated: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] -
Knockdown Validated: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - Non-transfected (-) and transfected (+) A549 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] -
Western Blot: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for CYP24A1 Antibody (HL1783) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYP24A1
Long Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Alternate Names
24-OHase, CYP24, EC 1.14.15.16
Gene Symbol
CYP24A1
Additional CYP24A1 Products
Product Documents for CYP24A1 Antibody (HL1783) - Azide and BSA Free
Product Specific Notices for CYP24A1 Antibody (HL1783) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...