CYP24A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52111

Novus Biologicals, part of Bio-Techne
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NBP1-52111
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CYP24A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RKYDIQATDN corresponding to internal region according to NP_000773.2, NP_001122387.1.

Epitope

C-RKYDIQATDN

Specificity

This antibody is expected to recognize both reported isoforms (NP_000773.2; NP_001122387.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CYP24A1 Antibody

Western Blot: CYP24A1 Antibody [NBP1-52111]

Western Blot: CYP24A1 Antibody [NBP1-52111]

Western Blot: CYP24A1 Antibody [NBP1-52111] - Analysis of CYP24A1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-52111 at 0.1 ug/m. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-52111]

Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-52111]

Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-52111] - 3.75ug/ml staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CYP24A1 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1-0.3 ug/ml
Application Notes
Western blot: Approx 50kDa band observed in Human and Rat Liver lysates (calculated MW of 51.2kDa according to NP_001122387.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Skin.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYP24A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system.

Long Name

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Alternate Names

24-OHase, CYP24, EC 1.14.15.16

Entrez Gene IDs

1591 (Human)

Gene Symbol

CYP24A1

UniProt

NP_000773.2 (Human)

Additional CYP24A1 Products

Product Documents for CYP24A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYP24A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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