CYP24A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85495

Novus Biologicals, part of Bio-Techne
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NBP1-85495
NBP1-85495-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYP24A1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP24A1 Antibody

Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495]

Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495]

Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Analysis in human urinary bladder and fallopian tube tissues using NBP1-85495 antibody. Corresponding CYP24A1 RNA-seq data are presented for the same tissues.
Western Blot: CYP24A1 Antibody [NBP1-85495]

Western Blot: CYP24A1 Antibody [NBP1-85495]

Western Blot: CYP24A1 Antibody [NBP1-85495] - Analysis in human cell lines A-549 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Immunocytochemistry/ Immunofluorescence: CYP24A1 Antibody [NBP1-85495]

Immunocytochemistry/ Immunofluorescence: CYP24A1 Antibody [NBP1-85495]

Immunocytochemistry/Immunofluorescence: CYP24A1 Antibody [NBP1-85495] - Staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.
View 4 more images

Applications for CYP24A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP24A1

The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is involved in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Long Name

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Alternate Names

24-OHase, CYP24, EC 1.14.15.16

Gene Symbol

CYP24A1

Additional CYP24A1 Products

Product Documents for CYP24A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYP24A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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