CYP24A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05981

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CYP24A1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CYP24A1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CYP24A1 Antibody

Western Blot: CYP24A1 Antibody [NBP3-05981]

Western Blot: CYP24A1 Antibody [NBP3-05981]

Western Blot: CYP24A1 Antibody [NBP3-05981] - Lane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa
Immunocytochemistry/ Immunofluorescence: CYP24A1 Antibody [NBP3-05981]

Immunocytochemistry/ Immunofluorescence: CYP24A1 Antibody [NBP3-05981]

Immunocytochemistry/Immunofluorescence: CYP24A1 Antibody [NBP3-05981] - Staining of CYP24A1 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CYP24A1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: CYP24A1 Antibody [NBP3-05981]

Immunoprecipitation: CYP24A1 Antibody [NBP3-05981]

Immunoprecipitation: CYP24A1 Antibody [NBP3-05981] - Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-CYP24A1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CYP24A1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size :59 kDa

Applications for CYP24A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP24A1

The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is involved in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Long Name

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Alternate Names

24-OHase, CYP24, EC 1.14.15.16

Gene Symbol

CYP24A1

Additional CYP24A1 Products

Product Documents for CYP24A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CYP24A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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