CYP27A1 Antibody (HL1820) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25429
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1820
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human CYP27A1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CYP27A1 Antibody (HL1820) - Azide and BSA Free
Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] -
Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] -
Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] -
Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for CYP27A1 Antibody (HL1820) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYP27A1
Long Name
Sterol 26-hydroxylase, mitochondrial
Alternate Names
CYP27, Cytochrome P450 27, EC 1.14.15.15
Gene Symbol
CYP27A1
Additional CYP27A1 Products
Product Documents for CYP27A1 Antibody (HL1820) - Azide and BSA Free
Product Specific Notices for CYP27A1 Antibody (HL1820) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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