CYP27A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32433

Novus Biologicals, part of Bio-Techne
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NBP2-32433
NBP2-32433-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYP27A1 Antibodies »

Product Specifications

Immunogen

This CYP27A1 Antibody was developed against a recombinant protein corresponding to amino acids: RQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP27A1 Antibody

Western Blot: CYP27A1 Antibody [NBP2-32433]

Western Blot: CYP27A1 Antibody [NBP2-32433]

Western Blot: CYP27A1 Antibody [NBP2-32433] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: CYP27A1 Antibody [NBP2-32433]

Immunocytochemistry/ Immunofluorescence: CYP27A1 Antibody [NBP2-32433]

Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody [NBP2-32433] - Staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-32433]

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-32433]

Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-32433] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells and in Langerhans islet cells.
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Applications for CYP27A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP27A1

Long Name

Sterol 26-hydroxylase, mitochondrial

Alternate Names

CYP27, Cytochrome P450 27, EC 1.14.15.15

Gene Symbol

CYP27A1

Additional CYP27A1 Products

Product Documents for CYP27A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYP27A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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