CYP46A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB400-140

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB400-140SS
NB400-140
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Rabbit

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CYP46A1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to residues between 200-250 of human CYP46A1. [UniProt# Q9Y6A2]

Reactivity Notes

Does not react with mouse.

Localization

Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP46A1 Antibody

Western Blot: CYP46A1 Antibody [NB400-140]

Western Blot: CYP46A1 Antibody [NB400-140]

Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.
Immunocytochemistry/ Immunofluorescence: CYP46A1 Antibody [NB400-140]

Immunocytochemistry/ Immunofluorescence: CYP46A1 Antibody [NB400-140]

Immunocytochemistry/Immunofluorescence: CYP46A1 Antibody [NB400-140] - CYP46A1 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: CYP46A1 Antibody [NB400-140]

Western Blot: CYP46A1 Antibody [NB400-140]

Western Blot: CYP46A1 Antibody [NB400-140] - Detection of Cyp46 in human brain lysates.
View 1 more images

Applications for CYP46A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000
Application Notes
This CYP46A1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot and Immunohistochemistry paraffin embedded sections. In ICC/IF endoplasmic reticulum staining was observed.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP46A1

Cholesterol homeostasis in brain relies on a unique catabolic pathway which involves neuronal specific CYP46A1 (cytochrome P450 46A1) mediated conversion of cholesterol into 24(S)-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol, the most important mechanism of cholesterol turnover. While cholesterol cannot pass blood brain barrier (BBB), the major cholesterol elimination product from brain tissues is 24S-hydroxycholesterol which has the ability to pass BBB into circulation via ABCA1 (the origin of more than 90 % of plasma 24S-hydroxycholesterol). CYP46A1 belongs to cytochrome P450 family of enzymes and it localize to ER/microsomes as single-pass membrane protein, and unlike other cytochrome P450 sterol hydroxylases that are predominantly expressed in the liver, CYP46A1 is highly expressed in neurons of several brain regions as well as of neural retina. CYP46A1 is involved in memory and cognition through its effect on cerebral cholesterol turnover and a single nucleotide polymorphism T/C (rs754203) in intron 2 of CYP46A1 has been discovered as responsible for increased susceptibility to Alzheimer's disease.

Alternate Names

CH24H, cholesterol 24-hydroxylase, CYP46CP46, Cytochrome P450 46A1, cytochrome P450, family 46, subfamily A, polypeptide 1, cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase), EC 1.14.13.98

Entrez Gene IDs

10858 (Human)

Gene Symbol

CYP46A1

UniProt

Q9Y6A2 (Human)

Additional CYP46A1 Products

Product Documents for CYP46A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CYP46A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.