CYP51A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48733

Novus Biologicals, part of Bio-Techne
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NBP2-48733
NBP2-48733-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYP51A1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQH

Reactivity Notes

Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP51A1 Antibody

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex, colon, liver and testis using Anti-CYP51A1 antibody NBP2-48733 (A) shows similar protein distribution across tissues to independent antibody NBP2-48793 (B).
Western Blot: CYP51A1 Antibody [NBP2-48733]

Western Blot: CYP51A1 Antibody [NBP2-48733]

Western Blot: CYP51A1 Antibody [NBP2-48733] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CYP51A1 Antibody [NBP2-48733]

Immunocytochemistry/ Immunofluorescence: CYP51A1 Antibody [NBP2-48733]

Immunocytochemistry/Immunofluorescence: CYP51A1 Antibody [NBP2-48733] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
View 6 more images

Applications for CYP51A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP51A1

CYP51A1, also known as Lanosterol 14-alpha demethylase, consists of a 56.8 kDa and a 46.3 kDa isoform and is involved in transforming lanosterol by removing the 14alpha-methyl group. Current research is being conducted with several diseases including tuberculosis, antley-bixler syndrome, colorectal cancer, leukemia, immunodeficiency, prostatitis, cholesterol, and chagas disease. This protein has been linked to the cholesterol and steroid biosynthesis, metabolism, and signaling pathways, where it interacts with FA2H, MSMO1, SCD, ZMPSTE24, and FDFT1.

Long Name

Lanosterol 14-alpha demethylase

Alternate Names

CYP51, CYPLI, Cytochrome P45014DM, Cytochrome P450LI, EC 1.14.14.154, LDM

Gene Symbol

CYP51A1

Additional CYP51A1 Products

Product Documents for CYP51A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYP51A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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