CYP7A1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04836
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 390-504 of human CYP7A1 (NP_000771.2). YPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CYP7A1 Antibody - Azide and BSA Free
![Western Blot: CYP7A1 AntibodyAzide and BSA Free [NBP3-04836]](https://resources.bio-techne.com/images/products/CYP7A1-Antibody-Western-Blot-NBP3-04836-img0001.jpg "Western Blot: CYP7A1 AntibodyAzide and BSA Free [NBP3-04836]")
Western Blot: CYP7A1 AntibodyAzide and BSA Free [NBP3-04836]
Western Blot: CYP7A1 Antibody [NBP3-04836] - Analysis of extracts of various cell lines, using CYP7A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.![Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836]](https://resources.bio-techne.com/images/products/CYP7A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04836-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836]")
Immunocytochemistry/ Immunofluorescence: CYP7A1 Antibody - Azide and BSA Free [NBP3-04836]
Immunocytochemistry/Immunofluorescence: CYP7A1 Antibody [NBP3-04836] - Analysis of HepG2 cells using CYP7A1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for CYP7A1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CYP7A1
Long Name
Cytochrome P450 7A1
Alternate Names
CP7A, CYP7, CYPVII
Gene Symbol
CYP7A1
Additional CYP7A1 Products
Product Documents for CYP7A1 Antibody - Azide and BSA Free
Product Specific Notices for CYP7A1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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