Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25430
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2144
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free
Immunogen
Recombinant fragment of human Cyr61/CCN1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free
Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -
Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -
Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:500. Blue: Fluoroshield with DAPI .
Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] -
Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cyr61/CCN1
Long Name
Cysteine-Rich, Angiogenic Inducer 61
Alternate Names
CCN1, GIG1, IGFBP-10
Gene Symbol
CCN1
Additional Cyr61/CCN1 Products
Product Documents for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free
Product Specific Notices for Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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