Cystathionase Antibody (JE35-91)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32240
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE35-91
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human Cystathionase aa 1-405 / 405. (Uniprot: P32929)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cystathionase Antibody (JE35-91)
Immunoprecipitation: Cystathionase Antibody (JE35-91) [NBP3-32240]
Immunoprecipitation: Cystathionase Antibody (JE35-91) [NBP3-32240] - Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with NBP3-32240 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32240 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: A431 cell lysate (input)
Lane 2: NBP3-32240 IP in A431 cell lysate
Lane 3: Rabbit IgG instead of NBP3-32240 in A431 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds
Western Blot: Cystathionase Antibody (JE35-91) [NBP3-32240]
Western Blot: Cystathionase Antibody (JE35-91) [NBP3-32240] - Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (NBP3-32240) at 1/2,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: 293T cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: HEK-293 cell lysate (20 ug/Lane)
Lane 5: NCI-H441 cell lysate (20 ug/Lane)
Lane 6: A431 cell lysate (20 ug/Lane)
Lane 7: F9 cell lysate (20 ug/Lane)
Lane 8: Human liver tissue lysate (40 ug/Lane)
Lane 9: Mouse liver tissue lysate (40 ug/Lane)
Lane 10: Rat liver tissue lysate (40 ug/Lane)
Lane 11: Human kidney tissue lysate (40 ug/Lane)
Lane 12: Mouse kidney tissue lysate (40 ug/Lane)
Lane 13: Rat kidney tissue lysate (40 ug/Lane)
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 8 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32240) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Cystathionase Antibody (JE35-91)
Application
Recommended Usage
Immunoprecipitation
1-2ug/sample
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cystathionase
Alternate Names
cystathionase (cystathionine gamma-lyase), cystathionine gamma-lyase, cysteine desulfhydrase, EC 4.4.1, EC 4.4.1.1, Gamma-cystathionase, homoserine deaminase, homoserine dehydratase, MGC9471
Gene Symbol
CTH
Additional Cystathionase Products
Product Documents for Cystathionase Antibody (JE35-91)
Product Specific Notices for Cystathionase Antibody (JE35-91)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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