Cystathionase Antibody (JE35-91)

Immunoprecipitation: Cystathionase Antibody (JE35-91) [NBP3-32240]

Immunoprecipitation: Cystathionase Antibody (JE35-91) [NBP3-32240] - Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with NBP3-32240 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32240 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: A431 cell lysate (input)
Lane 2: NBP3-32240 IP in A431 cell lysate
Lane 3: Rabbit IgG instead of NBP3-32240 in A431 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds

Western Blot: Cystathionase Antibody (JE35-91) [NBP3-32240]

Western Blot: Cystathionase Antibody (JE35-91) [NBP3-32240] - Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (NBP3-32240) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: 293T cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: HEK-293 cell lysate (20 ug/Lane)
Lane 5: NCI-H441 cell lysate (20 ug/Lane)
Lane 6: A431 cell lysate (20 ug/Lane)
Lane 7: F9 cell lysate (20 ug/Lane)
Lane 8: Human liver tissue lysate (40 ug/Lane)
Lane 9: Mouse liver tissue lysate (40 ug/Lane)
Lane 10: Rat liver tissue lysate (40 ug/Lane)
Lane 11: Human kidney tissue lysate (40 ug/Lane)
Lane 12: Mouse kidney tissue lysate (40 ug/Lane)
Lane 13: Rat kidney tissue lysate (40 ug/Lane)

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 8 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32240) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.