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Cystatin A Antibody (CPTC-CSTA-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07781

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CPTC-CSTA-1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Cystatin A protein (Uniprot: P01040)

Localization

Cell Surface, Cytoplasmic and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08498)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cystatin A Antibody (CPTC-CSTA-1)

Western Blot: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781]

Western Blot: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781]

Western Blot: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781] - Western Blot Analysis of THP-1 cell lysate using Cystatin A Mouse Monoclonal Antibody (CPTC-CSTA-1).
Immunohistochemistry-Paraffin: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781]

Immunohistochemistry-Paraffin: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781]

Immunohistochemistry-Paraffin: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cystatin A Mouse Monoclonal Antibody (CPTC-CSTA-1).
Immunohistochemistry-Paraffin: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781]

Immunohistochemistry-Paraffin: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781]

Immunohistochemistry-Paraffin: Cystatin A Antibody (CPTC-CSTA-1) [NBP3-07781] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cystatin A Mouse Monoclonal Antibody (CPTC-CSTA-1).

Applications for Cystatin A Antibody (CPTC-CSTA-1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cystatin A

Cystatin A, also known as Stefin A, Cystatin AS or Keratolinin, is a member of family 1 of the cystatin superfamily. It is a 98 amino acid, intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L.

Alternate Names

AREI, CSTA, Stefin A, STF1, STFA

Gene Symbol

CSTA

UniProt

Additional Cystatin A Products

Product Documents for Cystatin A Antibody (CPTC-CSTA-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cystatin A Antibody (CPTC-CSTA-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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