Cystatin A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86989

Novus Biologicals, part of Bio-Techne
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NBP1-86989
NBP1-86989-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cystatin A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cystatin A Antibody

Western Blot: Cystatin A Antibody [NBP1-86989]

Western Blot: Cystatin A Antibody [NBP1-86989]

Western Blot: Cystatin A Antibody [NBP1-86989] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Cystatin A Antibody [NBP1-86989]

Immunocytochemistry/ Immunofluorescence: Cystatin A Antibody [NBP1-86989]

Immunocytochemistry/Immunofluorescence: Cystatin A Antibody [NBP1-86989] - Staining of human cell line hTCEpi shows localization to nucleus & cytosol.
Immunohistochemistry-Paraffin: Cystatin A Antibody [NBP1-86989]

Immunohistochemistry-Paraffin: Cystatin A Antibody [NBP1-86989]

Immunohistochemistry-Paraffin: Cystatin A Antibody [NBP1-86989] - Staining of human skin shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
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Applications for Cystatin A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cystatin A

Cystatin A, also known as Stefin A, Cystatin AS or Keratolinin, is a member of family 1 of the cystatin superfamily. It is a 98 amino acid, intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L.

Alternate Names

AREI, CSTA, Stefin A, STF1, STFA

Gene Symbol

CSTA

Additional Cystatin A Products

Product Documents for Cystatin A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cystatin A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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