Cystatin F Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16074
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cystatin F. The exact sequence is proprietary.
Localization
Secreted; Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cystatin F Antibody
![Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074]](https://resources.bio-techne.com/images/products/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-16074-img0002.jpg "Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074]")
Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074]
Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074] - Analysis of paraffin-embedded CL1-5 xenograft, using cystatin F antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: Cystatin F Antibody [NBP2-16074] -
Immunocytochemistry/ Immunofluorescence: Cystatin F Antibody [NBP2-16074] - Cystatin F antibody detects Cystatin F protein at vesicle by immunofluorescent analysis.Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cystatin F stained by Cystatin F antibody (NBP2-16074) diluted at 1:500.Blue: Hoechst 33342 staining.
Western Blot: Cystatin F Antibody [NBP2-16074] -
Western Blot: Cystatin F Antibody [NBP2-16074] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin F antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for Cystatin F Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cystatin F
Alternate Names
CMAP, CST7, Cystatin 7, Leukocystatin
Gene Symbol
CST7
Additional Cystatin F Products
Product Documents for Cystatin F Antibody
Product Specific Notices for Cystatin F Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.