CYTB Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92639
![Immunohistochemistry-Paraffin: CYTB Antibody - Azide and BSA Free [NBP2-92639]](https://resources.bio-techne.com/images/products/CYTB-Antibody-Immunohistochemistry-Paraffin-NBP2-92639-img0002.jpg "Immunohistochemistry-Paraffin: CYTB Antibody - Azide and BSA Free [NBP2-92639]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CYTB (YP_003024038.1). RGLYYGSFLYSETWNIGIILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDLVQWIWGGYSVDSPTLTRFFTFHFILPFIIAALATLHLLFL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CYTB Antibody - Azide and BSA Free
![Western Blot: CYTB AntibodyAzide and BSA Free [NBP2-92639]](https://resources.bio-techne.com/images/products/CYTB-Antibody-Western-Blot-NBP2-92639-img0004.jpg "Western Blot: CYTB AntibodyAzide and BSA Free [NBP2-92639]")
Western Blot: CYTB AntibodyAzide and BSA Free [NBP2-92639]
Western Blot: CYTB Antibody [NBP2-92639] - Analysis of extracts of various cell lines, using CYTB at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.![Immunocytochemistry/ Immunofluorescence: CYTB Antibody - Azide and BSA Free [NBP2-92639]](https://resources.bio-techne.com/images/products/CYTB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92639-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CYTB Antibody - Azide and BSA Free [NBP2-92639]")
Immunocytochemistry/ Immunofluorescence: CYTB Antibody - Azide and BSA Free [NBP2-92639]
Immunocytochemistry/Immunofluorescence: CYTB Antibody [NBP2-92639] - Analysis of L929 cells using CYTB . Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: CYTB Antibody - Azide and BSA Free [NBP2-92639]](https://resources.bio-techne.com/images/products/CYTB-Antibody-Immunohistochemistry-Paraffin-NBP2-92639-img0003.jpg "Immunohistochemistry-Paraffin: CYTB Antibody - Azide and BSA Free [NBP2-92639]")
Immunohistochemistry-Paraffin: CYTB Antibody - Azide and BSA Free [NBP2-92639]
Immunohistochemistry-Paraffin: CYTB Antibody [NBP2-92639] - Human liver cancer using MT-CYB antibody at dilution of 1:100 (40x lens).Applications for CYTB Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CYTB
Alternate Names
cytochrome b, MTCYB, MT-CYB mitochondrially encoded cytochrome b
Gene Symbol
CYTB
Additional CYTB Products
Product Documents for CYTB Antibody - Azide and BSA Free
Product Specific Notices for CYTB Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov