Cytochrome b245 alpha Antibody (CS9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40974
![Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]](https://resources.bio-techne.com/images/products/Cytochrome-b245-alpha-Antibody-CS9-Flow-Cytometry-NBP1-40974-img0003.jpg "Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CS9
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
Specificity
This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Cytochrome b245 alpha Antibody (CS9)
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.![Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]](https://resources.bio-techne.com/images/products/Cytochrome-b245-alpha-Antibody-CS9-Flow-Cytometry-NBP1-40974-img0002.jpg "Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]")
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with LeucopermApplications for Cytochrome b245 alpha Antibody (CS9)
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Membrane permeabilisation is required for Flow Cytometry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytochrome b245 alpha
Alternate Names
cytochrome b light chain, Cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245 light chain, cytochrome b-245, alpha polypeptide, Cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, Neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, p22-PHOX, Superoxide-generating NADPH oxidase light chain subunit
Gene Symbol
CYBA
UniProt
Additional Cytochrome b245 alpha Products
Product Documents for Cytochrome b245 alpha Antibody (CS9)
Product Specific Notices for Cytochrome b245 alpha Antibody (CS9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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