Cytochrome b245 alpha Antibody (CS9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40974

Novus Biologicals, part of Bio-Techne
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NBP1-40974-0.1mg
NBP1-40974-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CS9

Concentration

1.0 mg/ml

View all Cytochrome b245 alpha Antibodies »

Product Specifications

Immunogen

Cytochrome B245 solubilised in octyl-beta-gluycopyranoside

Specificity

This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytochrome b245 alpha Antibody (CS9)

Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]

Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]

Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]

Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) - BSA Free [NBP1-40974]

Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm

Applications for Cytochrome b245 alpha Antibody (CS9)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Membrane permeabilisation is required for Flow Cytometry.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome b245 alpha

Alternate Names

cytochrome b light chain, Cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245 light chain, cytochrome b-245, alpha polypeptide, Cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, Neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, p22-PHOX, Superoxide-generating NADPH oxidase light chain subunit

Gene Symbol

CYBA

UniProt

P13498 (Human)

Additional Cytochrome b245 alpha Products

Product Documents for Cytochrome b245 alpha Antibody (CS9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytochrome b245 alpha Antibody (CS9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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