Cytochrome b245 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59573

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Cytochrome b245 alpha Antibodies »

Product Summary for Cytochrome b245 alpha Antibody

Immunogen

Synthetic peptides corresponding to CYBA(cytochrome b-245, alpha polypeptide) The peptide sequence was selected from the middle region of CYBA. Peptide sequence TILGTACLAIASGIYLLAAVRGEQWTPIEPKPRERPQIGGTIKQPPSNPP. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Cytochrome b245 alpha Antibody

Western Blot: Cytochrome b245 alpha Antibody [NBP1-59573]

Western Blot: Cytochrome b245 alpha Antibody [NBP1-59573]

Western Blot: Cytochrome b245 alpha Antibody [NBP1-59573] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.
Immunohistochemistry: Cytochrome b245 alpha Antibody [NBP1-59573]

Immunohistochemistry: Cytochrome b245 alpha Antibody [NBP1-59573]

Immunohistochemistry: Cytochrome b245 alpha Antibody [NBP1-59573] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Plasma membrane in intercalated disk Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec

Applications for Cytochrome b245 alpha Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome b245 alpha

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated

Alternate Names

cytochrome b light chain, Cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245 light chain, cytochrome b-245, alpha polypeptide, Cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, Neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, p22-PHOX, Superoxide-generating NADPH oxidase light chain subunit

Gene Symbol

CYBA

UniProt

P13498 (Human)

Additional Cytochrome b245 alpha Products

Product Documents for Cytochrome b245 alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytochrome b245 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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