Cytochrome b245 alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13323
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytochrome b245 alpha. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Cytochrome b245 alpha Antibody
![Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323]](https://resources.bio-techne.com/images/products/Cytochrome-b245-alpha-Antibody-Western-Blot-NBP3-13323-img0002.jpg "Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323]")
Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323]
Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323]](https://resources.bio-techne.com/images/products/Cytochrome-b245-alpha-Antibody-Immunohistochemistry-Paraffin-NBP3-13323-img0001.jpg "Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323]")
Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323]
Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323] - Cytochrome b245 alpha antibody detects Cytochrome b245 alpha protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cytochrome b245 alpha stained by Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Cytochrome b245 alpha Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytochrome b245 alpha
Alternate Names
cytochrome b light chain, Cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245 light chain, cytochrome b-245, alpha polypeptide, Cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, Neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, p22-PHOX, Superoxide-generating NADPH oxidase light chain subunit
Gene Symbol
CYBA
Additional Cytochrome b245 alpha Products
Product Documents for Cytochrome b245 alpha Antibody
Product Specific Notices for Cytochrome b245 alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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