Cytochrome b5 Outer Mitochondrial Membrane Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92276
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 16-150 of human CYB5B (NP_085056.2). KGQEVETSVTYYRLEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGHSSDAREMLKQYYIGDIHPSDLKPESGSKDPSKNDTCKSCWAYWILPIIGAVLLGFLYRYYTSESKSS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
![Western Blot: Cytochrome b5 Outer Mitochondrial Membrane AntibodyAzide and BSA Free [NBP2-92276]](https://resources.bio-techne.com/images/products/Cytochrome-b5-Outer-Mitochondrial-Membrane-Antibody-Western-Blot-NBP2-92276-img0001.jpg "Western Blot: Cytochrome b5 Outer Mitochondrial Membrane AntibodyAzide and BSA Free [NBP2-92276]")
Western Blot: Cytochrome b5 Outer Mitochondrial Membrane AntibodyAzide and BSA Free [NBP2-92276]
Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP2-92276] - Analysis of extracts of various cell lines, using Cytochrome b5 Outer Mitochondrial Membrane at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.Applications
Application
Recommended Usage
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytochrome b5 Outer Mitochondrial Membrane
Alternate Names
CYB5M, CYB5-M, CYPB5M, Cytochrome b5 outer mitochondrial membrane isoform, cytochrome b5 type B, cytochrome b5 type B (outer mitochondrial membrane), DKFZp686M0619, OMB5, outer mitochondrial membrane cytochrome b5, type 2 cyt-b5
Gene Symbol
CYB5B
Additional Cytochrome b5 Outer Mitochondrial Membrane Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov