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Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70544

Novus Biologicals, part of Bio-Techne
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NBP2-70544

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human CYP17A1(NP_000093) peoduced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

Western Blot: Cytochrome P450 17A1 Antibody (OTI3F11)Azide and BSA Free [NBP2-70544]

Western Blot: Cytochrome P450 17A1 Antibody (OTI3F11)Azide and BSA Free [NBP2-70544]

Western Blot: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544]

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544]

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544]

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544]

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free [NBP2-70544] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Applications for Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 17A1

The CYP17A1 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The CYP17A1 protein localizes to the endoplasmic reticulum. CYP17A1 has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in the CYP17A1 gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. (provided by RefSeq, Jul 2008)

Long Name

Steroid 17-alpha-hydroxylase/17,20 lyase

Alternate Names

CYP17, CYP17A1, CYPXVII, Cytochrome P450-C17, Cytochrome P450c17, EC 1.14.14.19, EC 1.14.14.32, S17AH

Gene Symbol

CYP17A1

Additional Cytochrome P450 17A1 Products

Product Documents for Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytochrome P450 17A1 Antibody (OTI3F11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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