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Cytochrome P450 1A2 Antibody (3D2)

Novus Biologicals, part of Bio-Techne | Catalog # H00001544-M10

Novus Biologicals, part of Bio-Techne
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H00001544-M10

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 3D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CYP1A2 (NP_000752, 211 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNL

Specificity

This product is specific for Human CYP1A2 monoclonal antibody (M10), clone 3D2 [Gene ID: 1544].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cytochrome P450 1A2 Antibody (3D2)

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (3D2) [H00001544-M10]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (3D2) [H00001544-M10]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (3D2) [H00001544-M10] - Analysis of monoclonal antibody to CYP1A2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Cytochrome P450 1A2 Antibody (3D2) [H00001544-M10] - Detection limit for recombinant GST tagged CYP1A2 is 0.3 ng/ml as a capture antibody.

Applications for Cytochrome P450 1A2 Antibody (3D2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 1A2

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Long Name

Cytochrome P450 1A2

Alternate Names

CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3

Gene Symbol

CYP1A2

Additional Cytochrome P450 1A2 Products

Product Documents for Cytochrome P450 1A2 Antibody (3D2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytochrome P450 1A2 Antibody (3D2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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