Cytochrome P450 2E1 Antibody (OTI5B9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02155

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunodiffusion, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5B9

Concentration

0.53 mg/ml

View all Cytochrome P450 2E1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 2E1 Antibody (OTI5B9)

Western Blot: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155]

Western Blot: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155]

Western Blot: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2E1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2E1.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2E1.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded Ovary tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.
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Applications for Cytochrome P450 2E1 Antibody (OTI5B9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.53 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 2E1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Alternate Names

4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

Entrez Gene IDs

1571 (Human)

Gene Symbol

CYP2E1

UniProt

P05181 (Human)

Additional Cytochrome P450 2E1 Products

Product Documents for Cytochrome P450 2E1 Antibody (OTI5B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome P450 2E1 Antibody (OTI5B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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