Cytochrome P450 2E1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52087

Novus Biologicals, part of Bio-Techne
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NBP1-52087
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cytochrome P450 2E1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RKVIKNVAEVK corresponding to internal region according to NP_000764.1.

Epitope

C-RKVIKNVAEVK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cytochrome P450 2E1 Antibody

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-52087]

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-52087]

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-52087] - Analysis of Cytochrome P450 2E1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-52087 at 0.05 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-52087]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-52087]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-52087] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Cytochrome P450 2E1 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.05 - 0.2 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human liver lysates (calculated MW of 56.8 kDa band according to NP_000764.1). IHC-P: Human liver shows ER-like staining in hepatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 2E1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Alternate Names

4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

Entrez Gene IDs

1571 (Human)

Gene Symbol

CYP2E1

UniProt

NP_000764.1 (Human)

Additional Cytochrome P450 2E1 Products

Product Documents for Cytochrome P450 2E1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 2E1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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