Cytochrome P450 2E1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85366

Novus Biologicals, part of Bio-Techne
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NBP1-85366
NBP1-85366-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytochrome P450 2E1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLIL

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24309898)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytochrome P450 2E1 Antibody

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366]

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366]

Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver shows strong cytoplasmic positivity in tumor cells.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85366 (A) shows similar protein distribution across tissues to independent antibody NBP1-85367 (B).
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Applications for Cytochrome P450 2E1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome P450 2E1

Alternate Names

4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

Gene Symbol

CYP2E1

Additional Cytochrome P450 2E1 Products

Product Documents for Cytochrome P450 2E1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome P450 2E1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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