Cytochrome P450 3A4 Antibody (3H8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37502
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3H8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human Cytochrome P450 3A4 expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytochrome P450 3A4 Antibody (3H8) - BSA Free
Western Blot: Cytochrome P450 3A4 Antibody (3H8)BSA Free [NBP2-37502]
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/ Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) - BSA Free [NBP2-37502]
Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) - BSA Free [NBP2-37502]
Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of human brain tissues using CYP3A4 mouse mAb with DAB staining.Applications for Cytochrome P450 3A4 Antibody (3H8) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYP3A4
Long Name
Cytochrome P450 3A4
Alternate Names
Albendazole Monooxygenase, Albendazole Sulfoxidase, CP33, CP34, CYP3A3, CYPIIIA4, NF-25, P450C3, P450PCN1, Quinine 3-Monooxygenase, Taurochenodeoxycholate 6-Alpha-Hydroxylase
Gene Symbol
CYP3A4
Additional CYP3A4 Products
Product Documents for Cytochrome P450 3A4 Antibody (3H8) - BSA Free
Product Specific Notices for Cytochrome P450 3A4 Antibody (3H8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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