Cytokeratin 1 Antibody (10W8J6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16883

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16883-20ul
NBP3-16883-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10W8J6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (P04264). GGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 1 Antibody (10W8J6)

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883]

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883]

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883]

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883]

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Cytokeratin 1 Antibody (10W8J6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 1

Belonging to the keratin family, Cytokeratin 1 plays an important role in cell binding. More specifically, this gene is expressed in the spinous and granular layers of the epidermis with keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. This gene also regulates the activity of kinases such as PKC and SRC by binding to ITGB1 and the receptor of GNB2L1. Other biological processes include complement activation, lectin pathway, epidermis development, fibrinolysis, regulation of angiogenesis and response to oxidative stress.

Alternate Names

CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1

Gene Symbol

KRT1

Additional Cytokeratin 1 Products

Product Documents for Cytokeratin 1 Antibody (10W8J6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 1 Antibody (10W8J6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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