Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48041
![Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted [NBP2-48041]](https://resources.bio-techne.com/images/products/Cytokeratin-10-13-Antibody-SPM262-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48041-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted [NBP2-48041]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM262
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium
Localization
Cytoplasmic
Specificity
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted [NBP2-48041]
Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted [NBP2-48041] - Human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (SPM262)Applications for Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 10/13
Alternate Names
BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, Keratin-10, KPP
Gene Symbol
KRT10
Additional Cytokeratin 10/13 Products
Product Documents for Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted
Product Specific Notices for Cytokeratin 10/13 Antibody (SPM262) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...